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Invest Ophthalmol Vis Sci 2003;44: E-Abstract 3777.
© 2003 ARVO

3777—B480

Prospective Variation in the Expression of the Conjunctival Mucin Genes Muc5ac, Muc7, Muc16, and Muc17 Before, During and After Contact Lens Wear

R.M. Corrales1, D.J. Galarreta1, J.M. Herreras1, M.J. González1, I. Arranz1, V. Saez1, A. Mayo1, C. García-Vázquez1, M. Calonge1 and F.J. Chaves2

1 Ioba, University of Valladolid, Valladolid, Spain
2 Endocrinology, Hospital Clínico Universitario, Valencia, Spain

Commercial Relationships: R.M. Corrales, None; D.J. Galarreta, None; J.M. Herreras, None; M.J. González, None; I. Arranz, None; V. Saez, None; A. Mayo, None; C. García-Vázquez, None; M. Calonge, None; F.J. Chaves, None.

Grant Identification: FIS-01/0185

Abstract

Purpose:Little is known about the potential of contact lens wear to induce a dry eye syndrome by altering the conjunctival mucins. This study analyze the variation of the conjunctival mucin genes expression published (MUC1, MUC2, MUC4, MUC5AC) in addition to others not published yet (MUC7, MUC15, MUC16, MUC17) in healthy subjects before, during, and after hydrogel contact lenses (HCL) wear for 1 year.
Methods:Two polyethersulfone filters were applied to the superior bulbar conjunctiva of both eyes of 8 healthy donors who never had worn contact lenses before, 6 months, and 1 year after HCL fitting, and 15 days after discontinuation. Real time PCR was performed using total RNA to quantite the expression of ocular mucin genes. Their relative quantity was determined by SYBR® Green I dye detection. The housekeeping gene GAPDH was used to normalize the amount of expression levels. Chronologic variations were analyzed using Student's test and lineal regression.
Results: Transcripts of MUC1, MUC2, MUC4, MUC5AC, MUC7, MUC15, MUC16, and MUC17 were detected in all healthy subjects just before HCL fitting. Changes in the expression of MUC1, MUC2, MUC4 and MUC15 were not significant after 6 or 12 months of HCL wearing. However, the number of transcripts for MUC5AC, MUC16, and MUC17 (p < 0.05) significantly decreased, whereas MUC7 significantly increased (p<0.0001) after 12 months of HCL wear. There were not significant changes in the expression of conjunctival mucin genes 15 days after discontinuing HCL wear.
Conclusions: This study is the first to report changes induced by HCL wear in a wide range of conjunctival mucin genes, also analyzing mucin genes not previously reported in the human conjunctival epithelium. These findings may offer additional etiopathogenic factors to be considered in the development of contact lens-induced dry eye syndrome.

Keywords: cornea: surface mucins • contact lens • conjunctiva

© 2003, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. For permission to reproduce any part of this abstract, contact the ARVO Office at arvo{at}arvo.org.




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