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The Toxicity of Trypan Blue and Indocyanine Green to Cultured Retinal Pigment Epithelial Cells

¹ Ophthalmology, University of MN, Minneapolis, MN, United States

Commercial Relationships: C.A. Cox, None; H.C. Chang, None; E.R. Steuer, None.

Grant Identification: no grants recieved

Abstract

Purpose:To determine the toxicity of trypan blue (TB) and indocyanine green (ICG) on cultured retinal pigment epithelial (RPE)cells, and to determine if light plays a role in toxicity.
Methods: Both trypan blue and indocyanine green of different concentrations were exposed to RPE cells for different time periods, either with or without light. Cytotoxicity was evaluated using the WST-1 colorimetric assay. The control was BSS exposed to the RPE cells either with or without light. The P value was pre-determined at <0.05. Analysis of Variance was performed.
Results:ICG at the higher concentration demonstrated toxicity (p<0.05) to the RPE cells when exposed to light. TB also showed toxicity to the cells in the presence of light (p<0.05). Statistical significance was also found when comparing ICG and TB to the RPE cells with light. ICG showed more toxicity than did trypan blue.
Conclusions:Exposure of cultured RPE cells to ICG and TB shows evidence for cytotoxicity in the presence of light.

Keywords: retinal pigment epithelium • stress response • clinical laboratory testing

© 2003, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. For permission to reproduce any part of this abstract, contact the ARVO Office at arvo{at}arvo.org.