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Transpupillary Thermotherapy (TTT) Induces Heat Shock Protein (HSP) 70 in the Rat Optic Nerve Head

Ophthalmology, Seoul National University College of Medicine, Seoul, Republic of Korea

Commercial Relationships: K. Park, None; J.M. Kim, None; H.J. Park, None; Y.J. Kim, None; D.M. Kim, None.

Support: BK21–2004

Abstract

Purpose: To investigate Hsp 70 induction in the optic nerve head after transpupillary thermotherapy(TTT) and to determine the optimum laser setting of Hsp 70 induction without tissue damage.

Methods:The 810 nm diode laser (Iridex Co., CA) was aimed to the center of optic nerve head of Norway brown rats with 50 um spot size. The various exposure power (range ; 20 – 200 mW) were used for, 60 seconds. And the various exposure duration (range; 1 minute – 5 minutes) were used for the same exposure power, 100mW. Left eyes were served as controls. Hsp 70 induction was examined with immunohistochemical staining. The structural change of the optic nerve head was further identified by confocal scanning laser ophthalmoscope and scanning electron microscopy (SEM).

Results: Induction of Hsp70 was observed in the optic nerve head at 100mW or above for 60 seconds. At the power of 140mW or above, photocoagulation of the optic nerve head was observed. In the confocal scanning laser ophthalmoscope and SEM findings, morphologic change of optic nerve head was started at the power of 140 mW and 120mW, respectively. Photocoagulation of the optic nerve head and peripapillary tissue was also found at 2 minutes and above with 100 mW.

Conclusions: Transpupillary laser irradiation of 100 mW power with 60 seconds induces Hsp 70 in the optic nerve head without tissue damage. The induction of Hsp 70 by TTT may provide a preliminary data for future neuroprotective experiments in glaucoma by enhancement of natural cytoprotective stress response.

Keywords: neuroprotection • ganglion cells • laser

© 2005, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. For permission to reproduce any part of this abstract, contact the ARVO Office at arvo{at}arvo.org.