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Invest Ophthalmol Vis Sci 2007;48: E-Abstract 5080.
© 2007 ARVO

5080—B228

The Outer Limiting Membrane as a Barrier to Photoreceptor Integration, After Transplantation to the Subretinal Space of the Adult Mouse

E. L. West1, R. A. Pearson1, J. C. Sowden2, R. E. MacLaren1 and R. R. Ali1

1Division of Molecular Therapy, Institute of Ophthalmology, University College London, United Kingdom
2Developmental Biology Unit, Institute of Child Health, University College London, United Kingdom

Commerical Relationships: E.L. West, None; R.A. Pearson, None; J.C. Sowden, None; R.E. MacLaren, None; R.R. Ali, None.

Support: Medical Research Council UK, Royal Blind Asylum and School, The Scottish National Institute for the War Blinded.

Abstract

Purpose:To determine whether the presence of the outer limiting membrane (OLM) in adult retinas limits the integration and differentiation of transplanted immature retinal cells.

Methods:A transient chemical disruption of the OLM was induced in adult mice. Cells from dissociated P1 neural retinas were transplanted into the subretinal space at various timepoints after chemical administration. At 3 weeks post injection, the number of integrated, differentiated photoreceptor cells present in the OLM disrupted eyes, was compared to the PBS-treated contralateral eye.

Results:The disrupted retina recovered normal morphology 2 weeks post chemical administration and the OLM reformed. A significant increase in cell integration was observed in the chemically disrupted, compared with PBS treated, eyes at the timepoint that corresponded to the peak of OLM disruption.

Conclusions:The presence of the OLM is a limiting factor with regard to photoreceptor transplantation in the adult mouse.

Keywords: retina • transplantation • photoreceptors

© 2007, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.




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