1Division of Molecular Therapy, Institute of Ophthalmology, University College London, United Kingdom
2Developmental Biology Unit, Institute of Child Health, University College London, United Kingdom
Commerical Relationships: E.L. West, None; R.A. Pearson, None; J.C. Sowden, None; R.E. MacLaren, None; R.R. Ali, None.
Support: Medical Research Council UK, Royal Blind Asylum and School, The Scottish National Institute for the War Blinded.
Purpose:To determine whether the presence of the outer limiting membrane (OLM) in adult retinas limits the integration and differentiation of transplanted immature retinal cells.
Methods:A transient chemical disruption of the OLM was induced in adult mice. Cells from dissociated P1 neural retinas were transplanted into the subretinal space at various timepoints after chemical administration. At 3 weeks post injection, the number of integrated, differentiated photoreceptor cells present in the OLM disrupted eyes, was compared to the PBS-treated contralateral eye.
Results:The disrupted retina recovered normal morphology 2 weeks post chemical administration and the OLM reformed. A significant increase in cell integration was observed in the chemically disrupted, compared with PBS treated, eyes at the timepoint that corresponded to the peak of OLM disruption.
Conclusions:The presence of the OLM is a limiting factor with regard to photoreceptor transplantation in the adult mouse.
Keywords: retina transplantation photoreceptors
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