ARVO Meeting Abstracts
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 QUICK SEARCH:   [advanced]


     


This Article
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Scoles, D.
Right arrow Articles by Dubra, A.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Scoles, D.
Right arrow Articles by Dubra, A.
Invest Ophthalmol Vis Sci 2013;54: E-Abstract 1434.
© 2013 ARVO


1434—B0161

In vivo microscopic inner retinal phenotypes of retinal and neurologic disease

Drew Scoles1, Robert Cooper2, Adam Dubis3, Brian Higgins4, Joseph Carroll4,5 and Alfredo Dubra4,6

1 Biomedical Engineering, University of Rochester, Rochester, NY
2 Biomedical Engineering, Marquette University, Milwaukee, WI
3 Ophthalmology, Duke University, Durham, NC
4 Ophthalmology, Medical College of Wisconsin, Milwaukee, WI
5 Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, WI
6 Biophysics, Medical College of Wisconsin, Milwaukee, WI

Commercial Relationships: Drew Scoles, None; Robert Cooper, None; Adam Dubis, None; Brian Higgins, None; Joseph Carroll, Imagine Eyes, Inc. (S); Alfredo Dubra, US Patent No: 8,226,236 (P)

Support: None

Abstract

Purpose:Reflectance/backscattering adaptive optics scanning light ophthalmoscopy (AOSLO) has been used extensively to study the healthy and diseased photoreceptor mosaic. With the exception of recent work on vascular imaging, the inner retina remains largely unexplored with this imaging modality. In this study, we investigate the potential of AOSLO for non-invasive study of the inner retina.

Methods:Inner-retinal AOSLO images from 60 patients representing over 24 different retinal diseases (see Table for full list), acquired over the period of three years were identified. All images were collected using 790 nm (13-15 nm FHWM bandwidth) light sources, on three similar AOSLO instruments. Subjects thought to be free of eye disease were also imaged for comparison.

Results:Inner retinal phenotypes were classified into 8 groups based on appearance, with most diseases demonstrating three or more distinct features. The two most common findings, punctate reflectivity and Gunn’s Dots were found in 88% and 54% of diseases respectively, as well as normal volunteers. Spectral domain optical coherence tomography, obtained on the day of AOSLO imaging in nearly all patients, did not identify all AOSLO findings.

Conclusions:Reflectance AOSLO imaging revealed a diverse set of inner-retinal phenotypes across these varied diseases. Interestingly, seemingly disparate diseases (e.g., post-operative macular hole and optic atrophy as well as commotio retinae and Best’s disease respectively) showed similar inner retinal findings. Common microscopic inner retinal phenotypes of retinal pathologies might point towards common pathways of disease or repair/defense mechanisms, and even suggest common treatment modalities.


Figure 01
View larger version (200K):
[in this window]
[in a new window]

 
A) Punctate reflectivity in rubella retinopathy. B) Gunn’s Dots in healthy volunteer. C) Granular reflectivity in Parkinson’s disease. D) Waxy reflectivity in cone-rod dystrophy. E) Abnormal nerve fiber layer in a patient with macular hole. F) Abnormal vessels in a patient with macular hole. G) Microcysts in dominant optic atrophy. H) Striate reflectivity in Best’s Disease. All scalebars 50 μm.

 

Figure 02
View larger version (51K):
[in this window]
[in a new window]

 
Retinal diseases and phenotypes observed in this study. Letters in column headers refer to the descriptions in the figure caption. The absence of a feature (empty square) should not be interpreted as an absolute absence, rather not yet found.

 

Keywords: 688 retina • 552 imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound)

© 2013, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.





HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH