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Invest Ophthalmol Vis Sci 2013;54: E-Abstract 6063.
© 2013 ARVO


In vivo imaging of Hypertensive Retinopathy using Fluorescence Adaptive Optics Scanning Light Ophthalmoscopy

Michael Dubow1,2, Alexander Pinhas1,2, Nishit Shah1, Yusufu Sulai3, Patricia Garcia1, Nicole Scripsema4, Joseph Carroll5, Alfredo Dubra5 and Richard Rosen1

1 New York Eye and Ear Infirmary, New York City, NY
2 Mount Sinai School of Medicine, Mount Sinai Hospital, New York City, NY
3 University of Rochester, Rochester, NY
4 New York Medical College, Valhalla, NY
5 Medical College of Wisconsin, Milwaukee, WI

Commercial Relationships: Michael Dubow, None; Alexander Pinhas, None; Nishit Shah, None; Yusufu Sulai, None; Patricia Garcia, None; Nicole Scripsema, None; Joseph Carroll, Imagine Eyes, Inc. (S); Alfredo Dubra, US Patent No: 8,226,236 (P); Richard Rosen, Opko-OTI (C), Optos (C), Clarity (C), OD-OS (C), Topcon (R), Zeavision (F), Genetech (F), Optovue (C)

Support: None


Purpose:While hypertension affects 1 in 3 adults and 2 in 3 adults over age 60, hypertensive retinopathy is often overlooked as an important indicator of systemic disease. Previous studies have explored the relationship between hypertensive retinopathy and diabetes, nephropathy, heart disease and cerebrovascular events. Reflectance adaptive optics scanning light ophthalmoscopy (RAOSLO) is a powerful tool in our exploration of the retina, providing access to retinal microstructure previously unavailable in vivo. However, the technology has not yet been optimized to study the microvasculature. Our group has combined AOSLO with fluorescein angiography (FAOSLO) to image patients with hypertensive retinopathy.

Methods:12 adult patients with vascular disease were recruited. Baseline RAOSLO images were collected using a 790 nm light source and 1° FOV. Patients then ingested 1g of fluorescein sodium mixed with juice. Starting at 15 minutes post ingestion, reflectance and fluorescence (488nm excitation emission; emission filter centered at 525nm; 45nm bandwidth) 1° FOV image sequences were recorded simultaneously. Conventional fundus photography with and without intravenous fluorescein was performed.

Results:FAOSLO revealed fine changes in the retinal microvasculature not seen with traditional imaging modalities and RAOSLO in all patients. We successfully imaged features of hypertensive retinopathy present in larger vessels on the microscopic scale, including microaneurysms, hemorrhages, intravascular lesions, focal arteriolar narrowing, and vessel tortuosity.

Conclusions:FAOSLO represents a promising new technology to image features of hypertensive retinopathy with resolution previously attainable only through biopsy. New quantitative classification schemas will need to be developed to describe and categorize these abnormalities. Following patients over time will capture the evolution of the disease, giving invaluable insight into the onset and progression of hypertensive changes, both in the eye and throughout the body.

Figure 01
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Figure 2: Variable vessel tortuosity was seen in all levels in hypertensive retinopathy.


Figure 02
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Figure 1: A tortuous tertiary artery imaged on both fundus photo (A) and IV fluorescein (B). While the artery was visible on RAOSLO (C), FAOSLO revealed a focal filling defect (D, E), suggesting local constriction or intravascular lesion.


Keywords: 552 imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • 688 retina

© 2013, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.